post-template-default single single-post postid-25009 single-format-standard

Controlling the Brain with Light

Alex Constantine - June 30, 2010

002692 10 fig1 300x295 - Controlling the Brain with LightBiomedical Optics & Medical Imaging

Controlling the brain with light
Ed Boyden | SPIE Newsroom | 10 June 2010

Ed Boyden is an assistant professor of biological engineering and brain and cognitive sciences at the MIT Media Lab. His award-winning research group develops tools to enable systematic understanding of neural circuits and discovers principles for controlling neural circuits to enable new treatments for intractable brain disorders.

Genetically encoded tools enable activation and silencing of neurons with light, revealing how the brain works and facilitating potential new therapies.

Our brains mediate everything we perceive, feel, decide, and do. This is accomplished by an incredibly densely packed network of hundreds of billions of neurons, which fall into perhaps hundreds of different classes, defined by their shape and the molecules they contain. Each computes in concert with thousands of others, its activity dynamically changing on the millisecond timescale. In many diseases, specific computations mediated by the brain are thrown into disarray when specific classes of neuron are compromised. Such neurological and psychiatric disorders affect billions of people worldwide and are some of the most intractable medical needs of our time. To treat these diseases, we need to understand how the elements of a neural circuit work together. This requires the ability to selectively perturb the activity of specific neurons within the circuit, observing the resultant impact on neural computation and behavior.

Until now, the ability to test the causal role of specific neuron types in emergent brain functions was limited. Lesions and pharmacological agents can eliminate the activity of neurons in a region, but the effects would apply to all cell classes in a region and would be long-lasting or even permanent, preventing researchers from analyzing when a given set of cells made its critical contribution. Stimulation of brain tissue with electrodes or magnetic fields enables temporally precise driving of neural activity, but such methods affect all neuron classes located within a region, including projection pathways entering or passing through the region. Accordingly, we have developed a series of molecular tools that enable activation or silencing of genetically specified neurons.

These are proteins encoded by genes from natural species of algae, fungi, and archaebacteria. These ‘optogenetic’ proteins can be thought of as tiny, genetically encoded solar panels: they sit, embedded, in the membranes of cells such as neurons and respond to light by altering the voltage of the cell. Since neurons are themselves electrical devices, using a virus to express these genes in neurons enables activation or silencing of the latter by different colors of light.

We have shown that the algal gene channelrhodopsin-2 (ChR2), which encodes a light-gated ion channel, can be inserted into neurons to activate them by brief pulses of blue light.1 In addition, my group has found that a halorhodopsin (Halo/NpHR), a light-gated chloride pump, can be used to silence neurons by yellow/red light.2 ChR2 and Halo can be used together in the same cell to activate and quiet neural activity with two different colors of light, enabling precise perturbations of the timing of electrical signals in the brain.3

More recently, we found a new reagent, nicknamed Arch (see Figure 1), that offers order-of-magnitude improvements in silencing power and recovery kinetics over halorhodopsins and enables near-digital switching off of neurons in the awake-behaving brain in response to yellow-green light.4 We also identified a reagent that we nicknamed Mac, a blue-light-driven neural silencer, opening up the ability to silence (alongside Arch or Halo) two different populations of neurons with different colors of light (see Figure 2).

002692 Big2 - Controlling the Brain with LightFigure 1. A neuron expressing the Arch gene and generating light-activated proteins that are then localized to the outside of the neuron, where they can control the neuron's activity in response to pulses of light. Scale bar: 20 μm.4

Note that these reagents can be expressed in practically any cell type. Given that cardiac, immune, pancreatic, and other kinds of cells can be electrically modulated, we can perform ‘synthetic physiology’ on these cells, controlling their state to assess how they contribute to organism or system-level functions.

002692 10 fig2 300x185 - Controlling the Brain with LightFigure 2. Demonstration of multicolor silencing of two different neurons. One neuron expresses Mac and is thus silenceable by blue but not by red light, while the second neuron expresses Halo and is thus silenceable by red but not by blue light.4

In summary, we have identified optogenetic proteins that act as molecular tools to make neurons controllable with pulses of colored light. We are now developing high-count arrays of optical fibers that enable perturbation of activity in distributed and complexly shaped neural circuits, in order to open up systematic analysis of brain circuits. By revealing the neural substrates that can most powerfully control and correct aberrant neural computations, these tools will lead to better therapies for treating brain disorders such as post-traumatic stress disorder, epilepsy, and Parkinson's disease. New drugs can be generated that target these control circuits but not other unrelated ones, presenting more efficacy and fewer side effects. They may also present new targets for neuromodulation therapies such as deep-brain and transcranial magnetic stimulation. The ability to optically control cells may directly enable a new generation of optical prostheses, which can precisely control aspects of patient physiology using temporally precise pulses of light.

ESB acknowledges funding by the National Institutes of Health (NIH) Director's New Innovator Award (DP2 OD002002-01), NIH Challenge Grant 1RC1MH088182-01, NIH Grand Opportunities Grant 1RC2DE020919-01, NIH 1R01NS067199-01, National Science Foundation (grants 0835878 and 0848804), the McGovern Institute Neurotechnology Award Program, the Department of Defense Congressionally Directed Medical Research Programs Post Traumatic Stress Disorder Program, the National Alliance for Research on Schizophrenia and Depression (since recently known by the business name NARSAD), the Alfred P. Sloan Foundation, Jerry and Marge Burnett, the Society for Neuroscience Research Award for Innovation in Neuroscience, the Massachusetts Institute of Technology Media Lab, the Benesse Foundation, and the Wallace H. Coulter Foundation.

Ed Boyden
Massachusetts Institute of Technology (MIT)
Cambridge, MA



1. E. S. Boyden et al., Millisecond-timescale, genetically targeted optical control of neural activity, Nat. Neurosci. 8, pp. 1263-1268, 2005.

2. X. Han, E. S. Boyden, Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution, PLoS ONE 2, pp. e299, 2007.

3. X. Han et al., Informational lesions: optical perturbation of spike timing and neural synchrony via microbial opsin gene fusions, Front. Mol. Neurosci. 2, pp. 12, 2009.

4. B. Y. Chow et al., High-performance genetically targetable optical neural silencing by light-driven proton pumps, Nature 463, pp. 98-102, 2010.


Leave a Reply

Your email address will not be published. Required fields are marked *